Analysis of Cyanazine and Atrazine in Cucumber by Pseudo Imprinted Solid-Phase Extraction Coupled with Liquid Chromatography

Abstract:

Two kinds of triazine herbicides (cyanazine and atrazine) in cucumber were analyzed by high performance liquid chromatography method coupled with pseudo imprinted solid-phase extraction (PISPE) as the clean-up arrays. The sorbent of PMISPE was synthesized by bulk polymerization using cyromazine as pseudo template and it could selectivly recognized cyanazine and atrazine from cucumber matrix. The mobile phase for separation and quantity analysis of cyanazine and atrazine was methanol-(0.8% acetic acid-water) (55:45, v/v) with a UV detector being set at 224 nm. The result showed that good linearity of the two trazine herbicides was obtained in a range of 0.004 µg/g ~ 0.400 µg/g with r2≥0.9996 and the recoveries at three spiked levels were ranged from 93.8% to 108.5% with RSD less than 3.4% (n=3).

• Periodical: Advanced Materials Research (Volumes 503-504)
• Pages: 289-292
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.503-504.289
• Online since: April 2012
• Authors: Hong Yuan Yan, Zhi Wei Li, Jing Yu Zhang, Chang Qing He
• Keywords: Atrazine, Cucumber, Cyanazine, Liquid Chromatography, Pseudo Imprinted Solid-Phase Extraction

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