Preliminary Purification and Distribution of Lipoxygenase in Grass Carp

Jian Hui Wang; Dong Min Liu; An Ni Zheng; Yong Le Liu; Qi Chen; Fa Xiang Wang; Xiang Hong Li; Jian Yu

doi:10.4028/www.scientific.net/AMR.550-553.1468

Paper Titles

Validation of Fermentative Parameters for Efficient Succinate Production in Batch Operation by Actinobacillus succinogenes 130ZT
p.1448

Screening and Influencing Factors of Halophilic Denitrifying Bacteria
p.1455

Stability of 4,4,4-Trifluoro-3-(indole-3-)butyric Acid Dissolved in Water on Growth of Rice Roots
p.1460

Comparison of 4,4,4-Trifluoro-3-(indole-3-)butyric Acid Dissolved in Water and Dimethyl Sulfoxide as a Solvent on Root Growth of Rice
p.1464

Preliminary Purification and Distribution of Lipoxygenase in Grass Carp
p.1468

Purification of Indoor Formaldehyde Using Ornamental Plant of Kafirlily
p.1473

Adsorptive Properties of the Preparation Material from the Skin of Corn Stalk for Enzyme Immobilization
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Characteristics and Flocculating Mechanism of a Bioflocculant M-1 Produced by Enterobacter sp. EP3
p.1482

Response Surface Method to Optimize Pretreatment of Rice Husk by Microwave-Assisted NaOH Solution
p.1486

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Preliminary Purification and Distribution of Lipoxygenase in Grass Carp

Abstract:

The lipoxygenase (LOX) in grass carp can catalyze the oxidation of polyunsaturated fatty acids even their esters, resulting in rancidity deterioration of grass carp and its products during the process and storage. Preliminary purification, quantitative determination and distribution of grass carp muscular LOX were investigated in the present study. LOX activity of the grass carp muscle was measured to be 35.17 KU/g and its molecular weight was 95.71 kDa identified by SDS-PAGE. Using previously reported extraction methods such as twice salting-out and once dialysis, the LOX protein content in the crude muscular LOX extraction is about 28%. When salted with graded saturation of ammonium sulfate, we found once salting-out was enough for LOX extraction and the effective percentage saturation of ammonium sulfate for LOX extraction was 40%. Under this condition, the LOX protein content in the crude muscular LOX extract was doubled. The LOX distribution in fish was gradually increased from skin and muscle, gill, viscera to blood, which indicates that it is very important to remove the viscera and bleed fish completely during fish product processing to decrease the risk of lipid oxidation caused by LOX.