Isolation and Purification of Aloin a and Aloin B by High-Speed Counter-Current Chromatography

Abstract:

High-speed counter-current chromatography(HSCCC) was successfully used for isolation and purification of Aloin A and Aloin B from the crude methanol extract of Aloe with a two-phase solvent system composed of chloroform–methanol–n-butylalcohol-water at an optimized volume ratio of 4:3:1:2 (v/v/v/v). The lower phase was used as the mobile phase in the head to tail elution mode. The preparative HSCCC separation was performed on 180 mg of the crude extract yielding pure Aloin A(18mg) and Aloin B(16mg) at purities of 95.2% and 96.8%, respectively, as determined by high performance liquid chromatography (HPLC). HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.

References

[1] E J Buenz. Toxicol. In. Vitro, Vol. 22(2008), p.422.

[2] M M Daud, M J Ibrahim, G R A Froemming, et al. The Breast, Vol. 20(2011), pS22.

[3] P K Mukherjee, N Maity, N K Nema, et al. Phytomedicine, Vol. 19(2011), p.64.

[4] A Vega-Gálvez, M Miranda, M Aranda, et al. Food Chem, Vol. 129(2011), p.1060.

[5] Y Ni, D Turner, K M Yates, et al. Int. Immunopharmacol, Vol. 4(2004), p.1745.

[6] L Chun-hui, W Chang-hai, X Zhi-liang, et al. Process Biochem, Vol. 42(2007), p.961.

[7] L Zhu, S Yu, X Zeng, et al. Sep. Purif. Technol, Vol. 63(2008), p.665.

[8] L Zhu, H Li, Y Liang, et al. Sep. Purif. Technol, Vol. 70(2009), p.147.

[9] F Yang, T Zhang, G Tian, et al. J. Chromatogr . A, Vol. 858(1999), p.103.

[10] H Cui, X Jia, X Zhang, et al. Sep. Purif. Technol, Vol. 77(2011), p.269.